Fig 1: Immunohistochemical detection of CK1δ in skeletal muscles of a six week old BALB/c mouse.Longitudinal section. Perfusion fixation with Bouin. Peroxidase reaction, dye: DAB. A similar CK1δ staining pattern of the myofibrils was detected independent of the antibody (NC10, 108, or ab10877). Specific antibody binding was visualised by the peroxidase reaction using DAB as substrate-chromogen. (A) NC10, immersion fixation with acid formalin; (B) 108, perfusion fixation with Bouin; (C): ab10877 (Abcam), immersion fixation with acid formalin. Magnification 100×. Using three different CK1δ specific antibodies only minor variations in the CK1δ staining pattern were observed. These could be explained by alterations in the phosphorylation status of CK1δ influencing the disposability of the particular antigenous epitope and/or by differences in the recognition of CK1δ splice variants being differently expressed in those cases.
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